| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brink, Keelyn R (10)
|
MRWISC |
44.96 |
42.13 |
-2.83 |
| 2 |
Zhang, Emma (9)
|
MRGATE |
54.11 |
43.55 |
-10.56 |
| 3 |
Pupke, Cameron R (10)
|
MRWISC |
43.50 |
43.79 |
0.29 |
| 4 |
Tse, Kaitlyn A (10)
|
MRSSC |
43.48 |
44.56 |
1.08 |
| 5 |
Singer, Keira A (10)
|
MRWISC |
50.77 |
45.10 |
-5.67 |
| 6 |
Greenberg, Ariel S (10)
|
MRSSC |
48.39 |
49.47 |
1.08 |
| 7 |
Duke, Ainsley C (9)
|
MRWISC |
49.84 |
50.91 |
1.07 |
| 8 |
Orner, Brooke E (9)
|
MRSSC |
57.47 |
58.76 |
1.29 |
| 9 |
Schneider, Holly P (10)
|
MRSSC |
58.00 |
1:00.37 |
2.37 |
|
Rose-Freaney, Cailyn G (8)
|
MRSSC |
1:00.19 |
1:00.52 |
0.33 |
|
Bove, Kaylee F (7)
|
MRSSC |
1:29.12 |
DQ |
- |